All-synchronized picosecond pulses and time-gated detection improve the spatial resolution of two-photon STED microscopy in brain tissue imaging

All-synchronized picosecond pulses and time-gated detection improve the spatial resolution of two-photon STED microscopy in brain tissue imaging

Blog Article

Super-resolution in two-photon excitation (2PE) microscopy offers case of caymus new approaches for visualizing the deep inside the brain functions at the nanoscale.In this study, we developed a novel 2PE stimulated-emission-depletion (STED) microscope with all-synchronized picosecond pulse light sources and time-gated fluorescence detection, namely, all-pulsed 2PE-gSTED microscopy.The implementation of time-gating is critical to excluding undesirable signals derived from brain tissues.

Even in a case using subnanosecond pulses for STED, the impact of time-gating was not negligible; the spatial resolution in the image of the brain tissue was improved by approximately 1.4 times compared with non time-gated image.This finding demonstrates that time-gating is more useful than previously thought for improving spatial resolution in brain tissue imaging.

This microscopy will facilitate deeper super-resolution observation of the fine structure of neuronal dendritic spines and car mackwell the intracellular dynamics in brain tissue.